Refining FlowCam Capture Settings for Accurate Particle Analysis

How to Determine the Right FlowCam Capture Settings for Your Samples

When it comes to flow imaging microscopy, data quality begins with your particle images. That's why  VisualSpreadsheet® software gives users the power to fine-tune image capture settings—ensuring accurate data collection across a wide range of sample types. Whether you're analyzing biotherapeutics, water samples, or high-performance materials, understanding and optimizing these settings is essential for reliable measurements of particle size, shape, and concentration.

Why Capture Settings Matter

Capture settings directly impact how particles are visualized and measured. Subtle changes can significantly affect the accuracy of your data, especially in applications where particle morphology and size distribution are critical. To support you in optimizing your capture settings, we offer a variety of resources for users just getting started with FlowCam, to those working on advanced method development.

Resources to Optimize Your Capture Settings

FlowCam 8000 Series Configuration Guide

Start with the correct setup. This guide walks you through configuring your FlowCam for the objective you plan to use, including recommended accessory pairings, particle size ranges, and fluidics settings. It also includes a basic set of capture settings for both phytoplankton and biopharmaceutical applications, making it easier to collect high-quality data from the start. 

Optimizing Capture Settings on FlowCam 8000 Series Instruments

Determine the best settings for your sample type. This technical note describes in detail how capture settings influence how particles are segmented from camera images and provides a step-by-step strategy for optimizing settings for specific applications.

File Processing Mode for Method Development

Need to adjust settings post-capture? Our guide to VisualSpreadsheet's File Processing Mode walks you through evaluating and further refining capture settings using previously collected data, perfect for method development and troubleshooting.

 

Additional Resources for Biopharma Applications

Recommended Capture Settings for Biopharmaceuticals 

This technical note provides a formal introduction to the recommended capture settings for biopharmaceutical applications, including examples of common particle types processed using these settings. This is a great place to start if you want to learn more about our recommended settings in these applications.

 

Unlock Better Data

By leveraging these tools and insights, you can dramatically improve the quality and reliability of your particle analysis data. Whether you're just getting started or refining an established method, our resources are here to help. 

Explore more technical content on our Resources page or through the FlowCam Customer Support Center

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