The Benefits of Light and Dark Pixel Thresholding

The Benefits of Light and Dark Pixel Thresholding

Thresholding is a method of image segmentation used in image processing to discern the boundaries of an object from its background. Semi-transparent particles, such as protein aggregates, are often mischaracterized or even undetected by most imaging particle analyzers when thresholding is missing or improperly configured.

Dark pixel thresholding, offered by most imaging particle analyzers, fails to discern particulate matter when it is lighter than the imaged background. Utilization of both dark and light pixel thresholding enables the detection of particulates expressing ranges in opacity (opaque to transparent) and improves the particle analyzer’s ability to detect, image, and analyze semi-transparent particles.

white-paper-thumbnail_the-benefits-of-light-and-dark-pixel-thresholdingIn our new white paper, we review the principles of dark and light pixel thresholding, and how these functions, along with VisualSpreadsheet’s neighborhood analysis, are necessary to accurately analyze semi-transparent particles. By understanding the issues of semi-transparent particle analysis and making it easy to optimize the system to account for them, FlowCam provides accurate particle characterization, count, and concentration.

Download White Paper



Post Topics

Related Posts

FlowCam printer toner circularity quality assurance
FlowCam Particle Analyzer for Printer Ink Pigment & Toner Quality Assurance
The uniformity of a printer toner particle affects the distribution of charge the particle holds and as a result, can affect the image quality of the …
Read Post
FlowCam instrument rendering on benchtop
What is Flow Imaging Microscopy?
Flow Imaging Microscopy is a fast and automated method to see highly-resolved digital images of microscopic particles in a flowing liquid. Using …
Read Post
Need Supplies?

Find supplies and spare parts for your FlowCam instrument or ask for a quote. 

Order Now

Need Help?

Get technical support and application help. Request training or preventative maintenance.

Submit a Support Ticket

Knowledge Base

Check out our collection of downloadable resources including white papers, application notes, technical notes, and videos.

View Resources