FlowCam® + LO (Light Obscuration)

FlowCam + Light Obscuration particle counter instrument

For the first time ever, you can obtain Light Obscuration data to meet regulatory guidelines, and also verify your data with real images, all with a single instrument, and one sample run. Our new FlowCam + LO instrument combines our patented flow imaging microscopy technology with an embedded light obscuration particle counter to provide you with the necessary data for USP regulations.

In the FlowCam + LO, a single sample passes continuously through two flow cells. The first uses FlowCam technology to capture digital images (Fig 1), and the second uses the light obscuration method to collect data for USP regulations. (Fig 2). 

Download the FlowCam + LO Brochure

 

 

How does it Work?

Micro Flow Imaging + embedded  light Obscuration module infographic

 

The Importance of Images

Given that Light Obscuration compares all particles to a sphere (equivalent spherical diameter or ESD), it's possible that extrinsic particles would have the same ESD as your proteins, and would pass the LO test. The image below shows the variety of particles you might see with a similar ESD (shown here: proteins, agglomerates, silicone droplets, air bubbles and other contaminants - silica, glass shards). A sample like this might pass USP size limits, but still fail at the trial stage. Verify your data with images, with the FlowCam + LO.

FlowCam 8000 Biopharma protein and inherent, intrinsic and extrinsic particles

FlowCam + LO Specifications:

  • Particle Size Range: 2 µm to 100 µm
  • Magnification: 10X (~100X magnification)
  • Flow Cells: Flow Imaging Module: 80 µm quartz, LO Module: 1 mm x 0.4 mm quartz
  • Sample Processing Capability: 0.2 mL / minute
  • Flow Imaging Module Camera: High resolution (1920 x 1200 pixels) CMOS (Monochrome and color available)
  • Frame Rate: Shutters up to 100 frames per second
  • Light Obscuration Light Source: Solid-state laser diode, 785 nm
  • Light Obscuration Detection Method: Light extinction, volumetric
  • Fluidics: Micro-syringe pump with 2.5 mL syringe
  • Equipped with image analysis software VisualSpreadsheet® 

Explore FlowCam Applications:

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Biopharma & Pharma

Water Quality Monitoring

Plankton Research

Harmful Algal Blooms

Food & Beverage

Industry