FlowCam® + LO (Light Obscuration)

FlowCam + LO instrument

Now, for the first time, you can obtain LO data to meet regulatory guidelines, and also verify your data with real images, all with a single instrument, and one sample run. Our new FlowCam + LO instrument combines our patented flow imaging microscopy technology with an embedded light obscuration module to provide USP 787/788 compliance.

In the FlowCam + LO, a single sample passes continuously through two flow cells. The first uses FlowCam technology to capture digital images (Fig 1), and the second uses a light obscuration module to provide data necessary for USP compliance (Fig 2). 

Download the FlowCam + LO Brochure

 

 

How does it Work?

Flow Imaging + LO Infographic with descriptions

 

The Importance of Images

Given that Light Obscuration compares all particles to a sphere (equivalent spherical diameter or ESD), it's possible that extrinsic particles would have the same ESD as your proteins, and would pass the LO test. The image below shows the variety of  particles you might see with a similar ESD (shown here: proteins, agglomerates, silicone droplets, air bubbles and other contaminants - silica, glass shards). A sample like this might pass USP size limits, but still fail at the trial stage. Verify your data with images, with the FlowCam + LO.

FlowCam 8000 Biopharm Collage 6.2.20 (2)

FlowCam + LO Specifications:

  • Particle Size Range: 2 µm to 100 µm
  • Magnification: 10X (~100X magnification)
  • Flow Cells: Flow Imaging Module: 80 µm quartz, LO Module: 1 mm x 0.4 mm quartz
  • Sample Processing Capability: 0.2 mL / minute
  • Flow Imaging Module Camera: High resolution (1920 x 1200 pixels) CMOS (Monochrome and color available)
  • Frame Rate: Shutters up to 100 frames per second
  • LO Light Source: Solid-state laser diode, 785 nm
  • LO Detection Method: Light extinction, volumetric
  • Fluidics: Micro-syringe pump with 2.5 mL syringe
  • Equipped with image analysis software VisualSpreadsheet® 

Learn More about FlowCam® Technology

Explore Aquatic Applications Explore Biopharma Applications Explore Industrial Applications

 

How Flow Imaging Microscopy Works >>

Benefits of Flow Imaging Microscopy >>

Explore our Applications:

Aquatic

Aquatic Research

Biopharm & Pharma

HAB Monitoring

Nanoparticles

Drinking Water

General Industry