Characterizing Protein Aggregation With Orthogonal and Complementary Analytical Techniques

Characterizing Protein Aggregation With Orthogonal and Complementary Analytical Techniques

A central challenge with particle analysis in biotherapeutics is the wide size range of particles that may be present, including particles from the active pharmaceutical ingredient (API), as well as excipients and contaminants. These particles can be as small as a few nanometers (e.g., oligomer-sized protein aggregates, individual viruses) or large enough to be seen by the unaided eye (e.g., visible API aggregates). While several analytical techniques exist for particle monitoring, each can only analyze particles within a finite size range.

Orthogonal and Complementary WP thumbnail
Download White Paper
In this white paper, we demonstrate how using a combination of orthogonal and complementary particle analysis techniques can give you a complete picture of the particles in your sample. We analyzed a simple protein formulation that was exposed to accelerated shaking and heat stress using four analytical techniques: subvisible flow imaging microscopy (FlowCam LO), light obscuration (FlowCam LO), submicron flow imaging microscopy (FlowCam Nano), and dynamic light scattering (DynaPro NanoStar). Additionally, this study used these particle measurement techniques to investigate the impact of surfactants on protein aggregation by performing the shaking and heat stress both with and without the presence of polysorbate 80.

 

Post Topics

Related Posts

FlowCam images of juice pulp
FlowCam Particle Analysis for Food and Beverage Characterization
When considering how to characterize your subvisible particles for food and beverage production it's important to be able to see the images of …
Read Post
FlowCam collage of oil and gas completion fluid particles
Better Cleanliness Testing of Hydraulic Fluid, Lubrication Oil, and Fuel with FlowCam
Hydraulic fluids, lubrication oils, and fuel need to be monitored for cleanliness according to standards such as ISO 4406 or NAS 1638. Optical …
Read Post
Need Supplies?

Find supplies and spare parts for your FlowCam instrument or ask for a quote. 

Order Now

Need Help?

Get technical support and application help. Request training or preventative maintenance.


Submit a Support Ticket

Knowledge Base

Check out our collection of downloadable resources including white papers, application notes, technical notes, and videos.

View Resources